Cisplatin stock solution
WebApr 10, 2024 · I have (100uM) stock solution in DMSO as (5mg/1850ul). I need to use 20um, 10um, 5um and 1um in working solution. How can I adjust DMSO concentration 0.1% in all of these solutions? WebJan 1, 1979 · Cisplatin reconstituted with 0.9% sodium chloride is stable for 24 hours at room temperature. It is not necessary to prepare doses immediately before administration, but the reconstituted drug should be protected from light. Refrigerated solutions of cisplatin should have a concentration less than 0.6 mg/ml in order to avoid precipitation.
Cisplatin stock solution
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WebMay 1, 1997 · Solutions stored in culture medium at -20 degrees C retained full cytotoxic potency. Conclusions: Cisplatin is a potent cytotoxic drug in vitro when stored under appropriate conditions. These findings are of relevance for in vitro studies with cisplatin. Publication types Research Support, Non-U.S. Gov't MeSH terms WebThe solubility of cisplatin in 0.9% NaCl is relatively low. The concentration of the stock solution is better less then 1 mg/ml (more preferably =0.5 mg/ml). Moreover, the …
WebNational Center for Biotechnology Information WebJun 5, 2002 · The recoveries of intact cisplatin and monohydrated cisplatin in plasma were determined by spiking blank plasma (in sixfold) with appropriate amounts of a 1 m M cisplatin stock solution to obtain concentrations of 300, 900 and 1800 n M intact cisplatin and 420, 700 and 2100 n M monohydrated cisplatin.
WebJul 2, 2013 · The stability of cisplatin hydrochloride standard stock solution in a mixture of 1.4 mg/mL of OPDA solution, phosphate buffer pH 6.8, and DMF at a concentration of 0.8 μ g/mL was investigated at different time intervals using the experimental conditions. 2.2.3. Validation of the Developed Method (1) Linearity and Range. WebOct 31, 2024 · Since the discovery of cisplatin, thousands of Pt and other platinum-group complexes have been synthesized and examined for their anticancer activity, both in …
WebDec 18, 2024 · Strikingly, we found that this inhibitory activity of cisplatin depends on a ligand exchange reaction which incorporates a solvent molecule (dimethylsulfoxide) into the complex, a modification...
WebMay 12, 2015 · Thought cisplatin can dissolve in DMSO and DMF, it can react with DMSO and DMF, forming adducts. It seems that cisplatin is stable in 0.9%NaCl solution, however, I get the information that... how many years ago was 2570 bceWebMar 9, 2024 · Aliquot the solution and store at −20 °C until use. Prepare the combination chemotherapeutics (combo) consisting of crizotinib plus cisplatin as follows: dissolve 5 mg of crizotinib in 1 mL solvent solution containing 25 μL of cisplatin stock solution to obtain a final concentration of 5 mg/mL crizotinib and 0.025 mg/mL cisplatin. how many years ago was 2400 bceWebCisplatin is soluble in DMF (approximately 16.6 mg/ml) with gentle heating. The cis-trans isomerization of cisplatin occurs in aqueous solution (in the absence of catalysts) and … how many years ago was buddhism foundedWebSep 19, 2016 · Cisplatin (CDDP) is an antineoplastic chemotherapy agent by cross-linking with DNA and causing DNA damage in cancer cells. Cisplatin activates ferroptosis and induces autophagy . For research … how many years ago was 3200 bcWebThe Tocris dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C 1, C 2 & V 2 to calculate V 1. Concentration 1 x Volume 1 = Concentration 2 x Volume 2 The dilution calculator equation The Tocris dilution calculator is based on the following equation: how many years ago was 300 bceWebCAS. 62996-74. Molecular weight. 466.54. Staurosporine is widely used as a positive control for inducing apoptosis. It has been proven that Staurosporine induces apoptosis in human malignant glioma cell lines, and arrests normal cells at the G1 checkpoint. Staurosporine is a potent, cell-permeable, reversible, ATP-competitive and broad spectrum ... how many years ago was 206 bcWebAll cisplatin stocks were stored and manipulated shielded from ambient light. The exact platinum concentrations of stock solutions were determined by inductively-coupled plasma atomic emission spectroscopic (ICP-AES) analysis. Prior to use, cisplatin aliquots were thawed at 37 °C and vortexed extensively. how many years ago was november 1998