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Phic31 integrase

WebMar 20, 2024 · The phiC31 integrase system, as a site-specific recombinase, could be a straightforward and efficient approach for this aim. According to the high potential of the application of phiC31 integrase system in stem cells, the present paper reviews previous studies, placing the focus on stem cell types and their application. WebpcDNA3.1 phiC31 was a gift from Konrad Basler (Addgene plasmid # 68310 ; http://n2t.net/addgene:68310 ; RRID:Addgene_68310) For your References section: An …

Addgene: pcDNA3.1 phiC31

WebIsolated from the bacteriophage PhiC31, the PhiC31 integrase (frequently also written as: ΦC31 integrase) encodes a serine-type recombinase that mediates the sequence-specific … http://www.systembio.com/wp/wp-content/uploads/phiC31_productsheet-1.pdf smite play 3 games in a party https://creativebroadcastprogramming.com

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WebFinally, the serine integrase PhiC31 was also applied in the yeast Saccharomyces cerevisiae as a marker recovery tool, indicating the … WebApr 1, 2010 · For systems using the φC31-integrase, the recognition sites on the PP are attB and attP sites (the bacterial and phage attachment sites of the φC31-integrase with approximately 40 bp each). WebThe phiC31 integrase system has, for the first time, enabled transformation of flies with BAC-sized constructs . The integrase can be expressed from mRNA co-injected with the construct or from the genome under a germ-line specific promoter, the latter method being more efficient . Several dozens of ... rite aid enumclaw wa 98022

Site-specific recombinase technology - Wikipedia

Category:Cloning, Expression, and in vitro Functional Activity Assay of phiC31 …

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Phic31 integrase

Pinpoint & PhiC31 Integrase Systems System Biosciences

WebPhiC31 integrase can integrate a plasmid of any size, as a single copy, and requires no cofactors. The integrated transgenes are stably expressed and heritable. SBI’s PhiC31 … WebJan 14, 2024 · PhiC31 integrase-mediated transgenesis systems are based on the site-specific bacteriophage PhiC31 integrase which mediates sequence-directed, irreversible and highly efficient integration between a bacterial attachment site ( attB) and a phage attachment site ( attP ).

Phic31 integrase

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http://www.hbaas.com/xumusuo/view/15912.html WebJun 19, 2014 · The ΦC31 system uses the properties of the site-specific integrase from the bacteriophage ΦC31, which mediates recombination between an attachment site ( attB) that is normally present in the...

WebJul 20, 2024 · We employed the PhiC31 integrase system to generate transgenic lines that permit overexpression of wild-type (WT) Drosophila TnT or the fly equivalent of the … WebpUPD2 PhiC31 integrase (GB1561) was a gift from Diego Orzaez (Addgene plasmid # 160586 ; http://n2t.net/addgene:160586 ; RRID:Addgene_160586) For your References section: A memory switch for plant synthetic biology based on the phage varphiC31 integration system.

WebThe phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two … WebThe use of phiC31 integrase requires a defined sequence for insertion and is compatible with insertion of a single copy of the transgene. We describe the protocol we use to facilitate phiC31 integrase transgene insertion including the use of insulator sequences to reduce position effect disruption of transgene activity.

WebAnti-Bacteriophage PhiC31 Integrase antibody Description Rabbit polyclonal to Bacteriophage PhiC31 Integrase Host species Rabbit Tested applications Suitable for: WB, ELISA more details Species reactivity Reacts with: Streptomyces phage phiC31 Immunogen

WebpUPD2 PhiC31 integrase (GB1561) was a gift from Diego Orzaez (Addgene plasmid # 160586 ; http://n2t.net/addgene:160586 ; RRID:Addgene_160586) For your References … smite photosWebJan 1, 2024 · So far, only the phiC31 integrase has been shown to work efficiently in Parhyale (Kontarakis et al., 2011) and a version of the Raeppli clonal marker which is … smite player count liveWebThe Jump-In™ technology helps accelerate stable cell line development due to the targeted integration of genetic material into specific integration sites using PhiC31 integrase. … smite play button not workingrite aid englewood flWebFeb 8, 2024 · The PhiC31 integrase system was further employed to optimize the CAR transduction and expression efficiency, which is encoded by a phage of streptomyces soil … smite player countWebFeb 12, 2015 · The integrase from phiC31 has been deployed in multiple eukaryotes, including fruit flies (Groth et al. 2004), zebrafish (Mosimann et al. 2013), tobacco (Lutz et al. 2004), and cultured human cells (Groth et al. 2000). Three components are required to integrate a transgene. rite aid ephrata pharmacyWebNational Center for Biotechnology Information rite aid enumclaw washington