Phospho-tag
WebJul 25, 2024 · Abstract Phos-tag gels are recent tools to dissect protein phosphorylation that operate by inducing a shift in the electrophoretic mobility of ... article describes the preparation and electrophoresis of Zn 2+-Phos-tag gels along with electrotransfer of the separated phospho- and nonphosphoproteins onto a PVDF membrane using either wet … WebJul 16, 2010 · Here we have optimized a Phos-tag-based system to detect the activation status of two proximal UPR sensors at the ER membrane. This method allowed for a quantitative assessment of the level of stress in the ER. ... Finally, in comparison to using commercially-available phospho-specific antibodies (e.g. P-Ser724A IRE1α and P-Thr980 …
Phospho-tag
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WebWe introduce two methods for the visualization of phosphorylated proteins using alkoxide-bridged dinuclear metal ( i.e. Zn 2+ or Mn 2+) complexes as novel phosphate-binding tag (Phos-tag) molecules. Both Zn 2+ - and Mn 2+ -Phos-tag molecules preferentially capture phosphomonoester dianions bound to Ser, Thr, and Tyr residues.
WebAug 17, 2024 · Here, we applied the Phos-tag SDS-PAGE technique to the analysis of GSK3β phosphoisotypes in cells and brains. There were three phosphoisotypes of GSK3β; double phosphorylation at Ser9 and... WebMar 20, 2024 · Phos-tag molecule can bind specifically to phosphate groups. • Phos-tag SDS-PAGE can separate phosphorylated and nonphosphorylated protein. • Phos-tag SDS-PAGE can profile phosphorylation status of protein kinases. epidermal growth factor receptor 2 extracellular signal-regulated kinase quantum dots 1. Introduction
WebPhospho-Tag™ Phosphoprotein Destain Solution is used for phosphoprotein gel stain to reduce nonspecific binding. The destain solution is prepared with sodium acetate buffer … Web这种可以“不依赖于环境”的方式识别被修饰残基的能力使得这些抗体具有广泛反应性，从而可能使它们能够与成百上千的不同蛋白发生反应。. 这种广泛的反应性模式使得这些抗体对多重分析和靶标发现项目尤为有价值。. 描述：Cell Signaling Technology 抗体剂型为 ...
WebPhospho-Tag™ Phosphoprotein Gel Stainis a high sensitive fluorescent stain designed for selectively detecting phosphoproteins in polyacrylamide gels. This stain contains a …
http://wolfson.huji.ac.il/purification/PDF/PAGE_SDS/Protein_Staining/PerkinElmer_PhostagGelStain.pdf crystal clear optical mcallenWebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse Monoclonal Antibody (CST #5282) is ready to ship. crystal clear operations ltdWebPhos-tag does not always work. No shift CANNOT exclude the possibility of protein phosphorylation. i have tested a couple of proteins that we have commercial phospho-specific antibodies for... crystal clear opticians southportWebApr 11, 2024 · Kras G12D induction slows the migration of myc-Ncl through the Phos-tag gel in an Erk1/2- and CK2-dependent manner. Myc-Ncl transfected iKras PDAC cells were grown in the absence of Dox for 48 h, before its addition to the indicated cells for a further 24 h, with or without Trametinib (10 nM) or Silmitasertib (10 μM). ... Phospho-motif ... dwarf cherry dazzle crape myrtle bushWebAs a control, we know that phosphorylation of a different kinase increases under the conditions we are testing (we can confirm with a phospho-specific antibody), but we have … crystal clear optometristWebMar 9, 2015 · The protein phosphorylation was assessed by phospho-tag gel (p-tag). ( b ) Schematic illustration of YAP protein domains and reported LATS kinase phosphorylation sites. The mutated serine sites in ... dwarf cherry tomato seedsWebPhosphate-binding tag, a new tool to visualize phosphorylated proteins We introduce two methods for the visualization of phosphorylated proteins using alkoxide-bridged dinuclear metal (i.e. Zn(2+) or Mn(2+)) complexes as novel … crystal clear optometry mcallen